Transcriptome-wide measurement of mRNA polyadenylation state
نویسندگان
چکیده
منابع مشابه
Transcriptome-wide analyses of CstF64-RNA interactions in global regulation of mRNA alternative polyadenylation.
Cleavage stimulation factor 64 kDa (CstF64) is an essential pre-mRNA 3' processing factor and an important regulator of alternative polyadenylation (APA). Here we characterized CstF64-RNA interactions in vivo at the transcriptome level and investigated the role of CstF64 in global APA regulation through individual nucleotide resolution UV crosslinking and immunoprecipitation sequencing and dire...
متن کاملTranscriptome-wide Regulation of Pre-mRNA Splicing and mRNA Localization by Muscleblind Proteins
The muscleblind-like (Mbnl) family of RNA-binding proteins plays important roles in muscle and eye development and in myotonic dystrophy (DM), in which expanded CUG or CCUG repeats functionally deplete Mbnl proteins. We identified transcriptome-wide functional and biophysical targets of Mbnl proteins in brain, heart, muscle, and myoblasts by using RNA-seq and CLIP-seq approaches. This analysis ...
متن کاملGenome-wide Compilation of mRNA Polyadenylation Signals in Arabidopsis Using Advance Searching Technologies
New computing technologies have recently become available to search and rapidly analyse large numbers of DNA sequences for critical information. Two technologies are of particular interest, general high-throughput sequencing libraries available on Cray systems such as the Cray SV1 and field-programmable gate arrays (FPGA) available as addons to such systems as the Sun Fire 6800. We have applied...
متن کاملMechanism and regulation of mRNA polyadenylation.
A poly(A) tail is found at the 38 end of nearly every fully processed eukaryotic mRNA and has been suggested to influence virtually all aspects of mRNA metabolism. Its proposed functions include conferring mRNA stability, promoting an mRNA’s translational efficiency, and having a role in transport of processed mRNA from the nucleus to the cytoplasm (for recent reviews, see Lewis et al. 1995; Sa...
متن کاملGenome-Wide Polyadenylation Maps Reveal Dynamic mRNA 3'-End Formation in the Failing Human Heart.
RATIONALE Alternative cleavage and polyadenylation (APA) of mRNA represents a layer of gene regulation that to date has remained unexplored in the heart. This phenomenon may be relevant, as the positioning of the poly(A) tail in mRNAs influences the length of the 3'-untranslated region (UTR), a critical determinant of gene expression. OBJECTIVE To investigate whether the 3'UTR length is regul...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Methods
سال: 2009
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2009.02.003